期刊论文

Hu, Z.-X.;Li, L.

中文

中国实验方剂学杂志

1005-9903

2022

28

9

117-124

10.13422/j.cnki.syfjx.20211775

国家自然科学基金项目（81774208）； 湖南省自然科学基金项目（2020JJ4062，2020JJ5408，2019JJ50447）； 湖南省中医药科研计划项目青年课题（2021180）；

本校为第一机构

目的:研究主动脉弓缩窄(TAC)致大鼠心力衰竭的病理过程与心肌组织代谢产物变化规律。方法:对大鼠进行TAC术,术后分为TAC-30 d组与TAC-60 d组,另设立同时段的假手术组作对照。对各组大鼠进行超声心动图和心肌组织病理染色;利用酶联免疫吸附测定法检测血清氨基末端脑钠肽前体(NT-proBNP)和三磷酸腺苷(ATP)含量;液相色谱-质谱联用技术观察心肌组织代谢产物与关联通路的变化特征,流动相水(A,含25 mmol·L~(-1)乙酸铵和25 mmol·L~(-1)氨水)-乙腈(B)梯度洗脱(0~0.5 min,95%B;0.5~7 min,95%~65%B;7~8 min,65%~40%B;8~9 min,40%B;9~9.1 min,40%~95%B;9.1~12 min, 95%B),电喷雾离子源,正、负离子检测模式...

Objective： To study the pathological process and changes of metabolites in myocardial tissue of heart failure induced by transverse aortic constriction（TAC）in rats. Method： Rats were treated with TAC operation and divided into TAC-30 d group and TAC-60 d group，and sham operation group at the same period was set up as control. Echocardiography and pathological staining of myocardial tissue were performed on rats in each group. Enzyme-linked immunosorbent assay was used to determine the expression of amino-terminal pro-brain natriuretic pept...