期刊论文

Rapid identification and content determination of hesperetin,nobiletin,naringin and neohesperidin in disinfectant containing extracts of citrus by TLC-HPLC-Q-TOF-MS

中文

分析试验室

1000-0720

2018

37

9

993-997

10.13595/j.cnki.issn1000-0720.2018.0192

81403206:国家自然科学基金 2014SK3042:湖南省科技厅社会发展项目 2014B131:湖南省教育厅优秀青年项目 K1406032-11:长沙市科技局重点项目 ZZGY17:中医药防治重症肝炎临床防治中心开放基金项目 ZZGY16:中医药防治重症肝炎临床防治中心开放基金项目

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建立了薄层色谱-高效液相色谱-四极杆飞行时间质谱法联用技术 (TLC-HPLC-QTOF-M S) 快速鉴定柑橘提取物消毒液中桔皮素、蜜橘黄素、新橙皮苷和柚皮苷4种活性物质并测定其含量的方法.样品经氯仿-丙酮洗脱剂梯度洗脱, TLC检识, 半制备HPLC纯化, HPLCM S/M S鉴定及HPLC含量测定.结果表明, 以氯仿-丙酮 (3:1, V/V) 作为展开剂分离效果最好.以甲醇-水 (7:3, V/V) 作为流动相测定桔皮素和蜜橘黄素的含量, 以甲醇-0.2％乙酸 (13:7, V/V) 作为流动相对柚皮苷和新橙皮苷进行定性和定量分析, 分离度及峰形最佳.方法测定桔皮素、蜜橘黄素、新橙皮苷和柚皮苷含量的相关系数均大于0.999, 检测限分别为:0.3, 0...

A new method based on TLC-HPLC-Q-TOF-MS was established to rapidly identify hesperetin, nobiletin, neohesperidin and naringin in disinfectant containing extracts of citrusand determinate their contents. The samples were gradiently eluted with chloroform-acetone, then identified by TLC, purified by semi-preparative HPLC, identified by HPLC-MS/MS and determined by HPLC. The results showed that the best conditions were as follows: unfolding agents (chloroform: acetone = 3: 1, V/V), mobile phase of determinating the contents of hesperetin and nobil...