期刊论文

中文

中国中西医结合杂志

1003-5370

2016

36

12

1496-1503

10.7661/CJIM.2016.12.1496

国家自然科学基金资助项目（No.81202647,81273670） 教育部博士点科研基金优先发展资助项目（No.20124323130001） 教育部博士点基金资助项目（No.20114323120001） 湖南省自然科学基金资助项目（No.12JJ4081） 湖南省优秀博士学位论文资助项目（No.YB2011B037） 中国博士后基金资助项目（No.2013M530355）

本校为第一机构

目的研究心血瘀阻证形成过程的代谢产物组学的变化。方法采用高脂饲料与冠状动脉左前降支结扎术结合的方法,制备大鼠心血瘀阻证形成的3个阶段。以血脂增高为血瘀证前期组,动脉粥样硬化形成为亚血瘀证期组,动脉粥样硬化基础之上结扎冠状动脉为心血瘀阻证组,每组8只。另取24只大鼠作为空白对照组,每个阶段8只。应用气相色谱-质谱联用仪(gas chromatography-mass spectrometer,GCMS),对不同组之间代谢产物含量的变化进行主成分分析(principal component analysis,PCA)及偏最小二乘法分析(partial least squares method,PLS)。结果 (1)在鉴定出的32中代谢产物中与心血瘀阻证形成过程密切相关依次是柠...

Objective To observe the changes of metabolomics in the evolution process of blockade of heart vessel syndrome (BHVS). Methods The formation of BHVS in three stages were sim- ulated by using high-fat forage and ligating the left anterior descending coronary artery. Increased blood lipid was in the early stage of blood stasis syndrome (BSS) group. Atherosclerosis (AS) was formed in the middle stage of BSS group (sub-BSS). Coronary artery was ligated on the basis of AS was the 3rd stage of BSS (BHVS group). There were 8 rats in each group. Totally 24 rats was used as the blank con- trol group an...