期刊论文

中文

中国老年学杂志

1005-9202

2014

34

5

1293-1295

10.3969/j.issn.1005-9202.2014.05.063

湖南省教育厅项目(No.10C1036) 湖南省“中药学”重点学科资助项目(湘教通〔2011〕76号)

本校为第一机构

目的 探讨中药复方滋肾益脑方对抑郁大鼠脑源性神经营养因子(BDNF)、酪氨酸激酶受体B(TrKB)基因表达的影响.方法 以采用灌胃甲状腺素合并慢性不可预见性应激制成肾阴虚抑郁动物模型后,随机分为正常组、模型组、氟西汀组、滋肾益脑方中、高剂量组,观察各组大鼠敞箱试验和液体消耗试验等行为学变化,采用RT-PCR法检测BDNF mRNA、TrKB mRNA表达.结果 与正常组比较,抑郁模型大鼠在敞箱试验、体重、糖水偏好试验等指标均存在显著性差异(P＜0.01),海马内BDNF mRNA、TrKB mRNA表达也显著降低.各药物组比较各指标均无统计学意义(P＞0.05),但滋肾益脑方高剂量组在改善抑郁行为(除糖水偏好外)、海马内BDNF ...

Objective To explore the effects of Zishen Yinao on the behavior and the expressions of brain-derived neurophic factor( BDNF) and its receptor tyrosine kinase receptors B( TrKB) in the hippocampus of depression model rats. Methods Combination of intragastric administration of thyroid hormone and chronic unpredictable stress were used to make kidney-yin deficiency model. Rats were randomly divided into normal,model,fluoxetine,middle and high dose of Zishen Yinao groups. The behavior of the open field test and liquid consumption,BDNF mRNA,TrKB mRNA expressions were detected. Results There were s...