期刊论文

中文

中国老年学杂志

1005-9202

2015

35

5

1307-1309

10.3969/j.issn.1005-9202.2015.05.074

81102593:国家自然科学基金 81273753:国家自然科学基金 No．201210541003:国家级大学生创新创业训练计划项目 :中西医结合基础湖南省重点学科建设项目

本校为第一机构

目的探讨左归降糖清肝方含药血清对脂肪酸孵育的人肝细胞株(HepG2)细胞内甘油三酯(TG)含量的影响。方法用混合脂肪酸孵育的HepG 2细胞建立细胞脂肪变性模型;将实验分为正常组,模型组,5%、10%、15%含药血清组,油红O染色法检测细胞内脂质沉积,同时定量检测细胞内TG含量。结果0.5 mmol/L 混合脂肪酸孵育HepG2细胞24 h,可诱导细胞内TG大量沉积,而10%、15% 左归降糖清肝方含药血清可抑制脂肪酸这一效应,使细胞内TG含量明显下降(P

Objective To investigate the effect of Zuogui Jiangtang Qinggan Recipe ( ZJQR) on triglyceride( TG) accumulation in HepG2 cells induced by fatty acid mixed.Methods The cell model of steatosis was established by fatty acid mixed in HepG2 cells.The ex-periments were divided into control, model, 5%, 10%, 15%medicated serum groups.The amount of TG in HepG2 cells was quantitatively analyzed by oil red O staining method.Results The fatty acid mixed (0.5 mmol/L) resulted in significant steatosis in HepG2 cells.The 10%, 15%medicated serum inhibited the effect of the fatty acid mixed, and significantly...