期刊论文

中文

中国老年学杂志

1005-9202

2019

39

23

5813-5817

10.3969/j.issn.1005-9202.2019.23.051

湖南省重点研发计划-国际与区域科技创新合作专题项目(2016WK2011) 湖南省教育厅科学研究项目(18A207) 2017年度湖南省大学生研究性学习和创新性实验计划项目(312)。

本校为第一机构

目的 探讨复方ANBP(由仙鹤草、藕节、乳香、蒲黄组成)促进全层皮肤缺损小鼠愈合的分子机制.方法 将C57BL/6小鼠随机分为空白对照组、ANBP组,每组随机分为6 h、3 d、7 d、14 d 4个时间点亚组,制备小鼠背部全层皮肤缺损模型.造模后空白对照组不做任何处理,ANBP组创面覆盖复方ANBP粉末.用免疫组织化学方法检测组织中转化生长因子(TGF)-β1、Sam和Mad同系物(Smad)2/3、Ski基因的蛋白表达量.结果 TGF-β1、Smad2/3的免疫组化结果显示:空白对照组从造模后6 h开始蛋白表达量逐渐升高,造模后7 d达到峰值,造模后14 d降低;ANBP组从造模后6 h开始蛋白表达量逐渐升高,造模后3 d达到峰值,造模后7～14 d蛋白表...

Objective To explore the molecular mechanism of compound ANBP on the healing of full-thickness skin defect. Methods C57 BL/6 mice were randomly divided into blank control and ANBP groups, and each group was randomly divided into four time points of 6 h, 3 d, 7 d and 14 d to prepare the full-thickness skin defect model of mouse back. No treatment was done in blank control group, and the wound was covered with compound ANBP powder in ANBP group. The protein expressions of TGF-β1, Smad2/3 and Ski in tissues were detected by immunohistochemistry, ...