期刊论文

Proteomic features of nasopharyngeal tissue sample of NPC familial patients in different constitutional patterns determined by 2-Dimensional electrophoresis

中文

中国中西医结合耳鼻咽喉科杂志

1007-4856

2010

18

1

4-7

10.3969/j.issn.1007-4856.2010.01.002

30572408,30672738,30973856:国家自然科学基金 2050002:广东省中医药科研项目 06WK3016:湖南省科技厅国际科技合作项目 07JJ6041:湖南省自然科学基金

本校为第一机构

目的 建立鼻咽癌高癌家系鼻咽癌患者气虚体质证犁及湿热体质证型高分辨率双向凝胶电泳特征图谱,探讨其证本质相关性蛋白质组学特征.方法 设鼻咽癌高癌家系鼻咽癌患者气虚体质组、湿热体质组、其他体质证型组,散发性鼻咽癌患者组,鼻明癌高癌家系健康成员对照组,共计5组.分别获取鼻咽组织标本,提取总蛋白质,固相pH梯度双向凝胶电冰分离,经考马斯亮蓝显色后,Image Master 2D Elite4.01图像分析软件进行电泳结果分析.结果 各组标本均获得分辨率高、重复性好的双向凝胶电泳图谱,平均蛋白质点数分别为860±20,854±25,842±30,867±20及857±15.比较分析,发现鼻咽癌高癌家系鼻咽癌患者气虚体质组与湿热...

Objective To identify the characteristics of high resolution 2-dimensional electrophoregrams of the total protein separated from nasopharyngeal focus tissues of cases with nasopharyngeal carcinoma （NPC） in either constitutional pattems of Qi deficiency or dampness-heat in familial NPC to explore its essential qualities of constitutional patterns in terms of proteomics. Methods Included in this study were 5 groups of eases in total, i.e. 3 groups of NPC eases with the constitutional pattern of Qi deficiency, dampnessheat and the mixed group of...