期刊论文

中文

湖南中医杂志

1003-7705

2014

30

8

152-154

10.16808/j.cnki.issn1003-7705.2014.08.078

81273990:国家自然科学基金 2013000011:高等学校博士学科点专项科研基金(博导类) 1300001:湖南省中医药管理局重点资助项目

本校为第一机构

目的:观察七味白术散对轮状病毒(HRV)、柯萨奇B3病毒(Cox B3)感染HT-29细胞干扰素诱导蛋白-10(IP-10)表达的影响.方法:将HT-29细胞株分别随机设成正常组、模型组、七味白术散组、病毒唑组,于37℃、5％ C02条件下培养48h,收获培养物.采用RT-PCR技术测定IP-10mRNA的表达情况.结果:两种肠道病原体感染HT-29细胞后,与正常组比较,模型组IP-10mRNA的表达明显升高(P＜0.05);与模型组比较,七味白术散组和病毒唑组的IP-10mRNA表达明显降低(P＜0.05);七味白术散组表达值低于病毒唑组(P＜0.05).结论:七味白术散能下调HRV、COX B3感染HT-29细胞的IP-10的表达.

Objective：To observe the effect of Qiwei Baizhu powder on the expression of interferon - inducible protein 10 （ IP - 10） in HT - 29 cells infected with human rotavirus （HRV） and coxsaekievirus B3 （ Cox B3 ）. Methods ： HT - 29 ceils were randomly divid- ed into normal group, model group, Qiwei Baizhu powder group, and virazole group and were cultured at 37~C and 5% CO2 for 48 h. The nlRNA expression of IP - 10 was measured by RT - PCR. Results：After being infected with the two enteric pathogens,the model group had significantly increase...