期刊论文

中文

赣南医学院学报

1001-5779

2013

04

488-491

基金项目：国家林业公益性行业科研专项“山银花、厚朴优良新品种创制及利用技术研究”（201104023）

本校为第一机构

目的:优化山银花中绿原酸提取工艺。方法:将山银花甲醇回流前,用果胶酶、纤维素酶分别处理,与未加酶组比较,得出最优酶,探讨最优酶的用量、时间、温度、pH对绿原酸得率的影响。结果:果胶酶较纤维素酶提取绿原酸得率高,最优酶提取最佳条件为:加入pH 4.5果胶酶0.5%、在50℃酶解1.5 h,再在70℃浸提2次/1 h,其绿原酸含量为2.87%。结论:酶法提取山银花能提高绿原酸得率。

Objective：To optimize the extraction process of chlorogenic acid in Flos Lonicerae Confusae. Method：The Flos Lonicerae Confusae was treated by means of pectase, cellulase, respectively, before extracted by methanol reflux, compara- tive negative control i studying the optimized enzyme dosage, treatment time, treatment temperature and pH how to influence the yield of chlorogenic acid. Result：The chlorogenic acid yield of pectase enzymatic hydrolysis was higher than cellulase. The optimum dosage was 0.5% ,pH ：4.5 ,time of treatment was 1.5 h,...