Objective: This study was conducted to develop an method for identification of Trachemys scripta elegans (Ba Xi Gui, 巴西龟) shell glue from Chinemys reevesii (Zhong Hua Cao Gui, 中华草龟) shell glue. Methods: In this research, an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method coupled with Binary Compare tool of UNIFI software was validated to differentiate Trachemys scripta elegans (Ba Xi Gui, 巴西龟) shell glue from Chinemys reevesii (Zhong Hua Cao Gui, 中华草龟) shell gl...