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Facile combination of beta-cyclodextrin host-guest recognition with exonuclease-assistant signal amplification for sensitive electrochemical assay of ochratoxin A

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成果类型:
期刊论文
作者:
Wang, Yonghong;Ning, Ge;Wu, Yaohui;Wu, Shun;Zeng, Baiquan;...
通讯作者:
Liu, Gaoqiang
作者机构:
[Liu, Gaoqiang; Wu, Yaohui; Zeng, Baiquan; Wu, Shun; Wang, Yonghong] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Coll Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.
[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Hunan, Peoples R China.
[He, Xiaoxiao; Wang, Kemin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Biol, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.
通讯机构:
[Liu, Gaoqiang] C
Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Coll Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.
语种:
英文
关键词:
Electrochemical;Ochratoxin A;Host-guest recognition;RecJf exonuclease;Signal amplification
期刊:
Biosensors and Bioelectronics
ISSN:
0956-5663
年:
2019
卷:
124-125
页码:
82-88
基金类别:
Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2018JJ3869]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21305164, 31772374]; Research Foundation of Education Bureau of Hunan Province, China [16A227]; National Key R&D Programs of Intergovernmental International Science and Technology Cooperation [2017YFE0108100]
机构署名:
本校为其他机构
院系归属:
国际教育学院
摘要:
Smartly coupling exonuclease-induced target recycling signal amplifications with β-cyclodextrin host-guest recognition, a novel “signal-on” aptamer sensor for sensitive determination of ochratoxin A (OTA) was proposed for the first time. Firstly, the formation of double-strand DNA (dsDNA) was occurred by hybridizing OTA aptamer with its complementary DNA (cDNA) and as the probe DNA the cDNA at its 3′ terminal was labeled with methylene blue (MB). Next, when OTA was present, the aptamer tended to form aptamer-OTA complex with conformation of...

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