期刊论文

Effect of Kidney-tonification, Spleen-invigoration and Detoxification Methods on T iymphocytes Mediated by Peripheral Blood DCs in Chronic HBV Infection Patients

中文

世界科学技术-中医药现代化

1674-3849

2013

5

825-830

10.11842/wst.2013.05.005

(JDZX2012061):国家中医药管理局国家中医临床研究基地业务建设科研专项 :“阳黄-阴阳黄-阴黄”辨证论治模式对乙型肝炎相关性肝衰竭的干预作用及其预后的影响，负责人孙克伟 (81072779/HZ708):国家自然科学基金委面上项目 :HBV慢性感染过程不同阶段扶正与祛邪法干预的细胞免疫学机理研究，负责人孙克伟

本校为第一机构

目的：比较健脾、补肾与解毒法对慢性乙型肝炎患者外周血DCs介导T淋巴细胞的影响。方法：分别收集10例HBV慢性乙型肝炎患者和8例健康者外周血，分离外周血单个核细胞（PBMCs），体外培养DCs ，用大鼠健脾、补肾、解毒药物血清进行干预，并建立DCs、T细胞相互作用的模型，检测T细胞的表面分子CD3+ T细胞、CD4+ T细胞、CD8+ T细胞、CD8+CD28+ T细胞、CD8+ T细胞PD-1受体的表达，并检测T细胞上清液中IFN-酌的分泌水平。结果：经补肾、健脾、解毒药物血清处理，HBcAg负载DCs孵育的T淋巴细胞，CD3+ T细胞、CD4+ T细胞表达率升高，CD8+ T细胞、CD8+T细胞PD-1表达率减低，IFN-酌水平提高，六味地黄丸组...

This study was aimed to compare the effect of traditional Chinese medicine ( TCM ) treatment method of kidney-tonification, spleen-invigoration and detoxification on T lymphocytes of patients with chronic HBV infec-tion, which were mediated by peripheral blood dendritic cells (DCs). Ten patients with chronic HBV infection and eight healthy controls were included in this study. DCs were differentiated from PBMCs which were isolated from their peripheral blood, incubated and induced. Then, the DCs were interfered by plasma of medicine through the...