期刊论文

Identification of Pig-Hide Gelatin from Tortoise-Shell Glue by UPLC-ESI-QTOF-MS/MS

中文

世界科学技术-中医药现代化

1674-3849

2017

19

2

339-343

10.11842/wst.2017.02.024

国家中医药管理局“药用植物学”重点学科课题（国中医药发[2009]30号）,负责人:李顺祥； 湖南省教育厅“中药学”重点学科（湘教通[2011]76号）,负责人:李顺祥;湖南省教育厅高校科技创新团队（湘教通[2010]212号）,负责人:李顺祥；

本校为第一机构

目的:检测龟甲胶中新阿胶特征肽成分。方法:采用胰蛋白酶进行酶解,利用超高效液相色谱-电喷雾四极杆飞行时间质谱法(UPLC-ESI-QTOF-MS/MS)对龟甲胶样品进行测定,选定新阿胶特征肽离子m/z 925.4作为检测离子。结果:龟甲胶特征成分含量偏低的样品(但不含有牛皮元和驴皮元特征肽成分)中检测出新阿胶特征肽离子m/z 925.4信号,其MS/MS裂解规律与文献报道一致,而龟甲胶标准品中未检测出该特征肽信号。结论:该方法专属性强、灵敏度高,可检测出龟甲胶中是否掺杂新阿胶成分,可用于龟甲胶的质量控制。

This study aimed at identifying pig- hide gelatin from tortoise shell glue using ultra- performance liquid chromatography-electrospray-time of flight mass spectrometry(UPLC-ESI-QTOF-MS/MS).The samples of tortoise shell glue were digested by trypsin before the analysis on UPLC coupled with QTOF mass spectrometry. The ions at m/z 925.4 were selected as the marker peptides of pig-hide gelatin. It was found that the test results of the ions at m/z 925.4 were positive in the samples with low contents of the marker peptides of tortoise shell glue, indicating the existence of pig-hide gelatin. Howeve...