期刊论文

Comparative Analysis of Amino Acid Composition between Granules and Traditional Paste of Guilu Erxian JiaoFormula and Their Effect on Serum Levels of Immunoglobulin in Rats

中文

世界科学技术-中医药现代化

1674-3849

2021

23

05

1397-1405

10.11842/wst.20200717002

三九集团资助横向课题（50010151）：中药配方颗粒制膏研究，负责人：何清湖;三九集团资助横向课题（50010152）：传统龟鹿二仙膏与颗粒剂龟鹿二仙膏对阳虚质小鼠的影响，负责人：何清湖； 东健集团资助横向课题（2019M652775）：龟甲胶的指纹图谱及液质分析研究,负责人：余娜；

本校为第一机构

目的 对比龟鹿二仙胶配方颗粒膏与传统饮片膏中的13种氨基酸类成分以及二者对大鼠血清免疫球蛋白的影响.方法 采用柱前衍生-UPLC法分析19批龟鹿二仙胶配方颗粒膏与传统饮片膏样品,建立二者的指纹图谱,并采用标准品对照,对其中氨基酸成分进行定性分析,并取其中含量最高的几种成分进行定量分析.建立大鼠阳虚型模型,分别采用龟鹿二仙胶配方颗粒膏与传统饮片膏进行干预,检测各组大鼠血清中IgA、IgG、IgM免疫球蛋白的含量.结果 成功建立龟鹿二仙颗粒膏与饮片膏的指纹图谱,发现19批膏方的相似度接近(P>0.99),采用标准品确定其中13种氨基酸成分,其中各批次中成分含量较高的4种氨基酸,L-羟脯氨酸、甘氨酸...

Objective To compare the amino acid components in Guilu Erxian Jiaopaste and granules and their effects on serum levels of immunoglobulin in rats.Methods Pre-column derivatization-UPLC was used to analyze 19 batches of samples, the fingerprints of Guilu Erxian Jiao paste and granules were established, and analyze the amino acid composition and take quantitative analysis of several components with the highest content.A model of rats with yang deficiency was established, and Guilu Erxian Jiao paste and granules were used as interventions to detect the content of IgA, IgG and IgM immunoglobulin i...