期刊论文

Effects of MEBT/MEBO on the expression of TGF-β1,Smad3 and P-smad3 and morphological structure in diabetic foot wound of rats

中文

重庆医科大学学报

0253-3626

2017

42

3

283-288

10.13406/j.cnki.cyxb.001179

2015年国家自然科学基金资助项目（编号:81560776）； 2014年广西自然科学基金面上资助项目（编号:2014GXN SFAA118135）；

本校为其他机构

目的:观察皮肤原位再生医疗技术(moist exposed burn therapy/moist exposed burn ointment,MEBT/MEBO)对大鼠糖尿病足创面组织转化生长因子-β1(transforming growth factor-β1,TGF-β1)、Smad3、磷酸化smad3(P-smad3)表达及形态学结构的影响。方法:将80只健康雄性SD大鼠随机分为空白对照组、模型组、贝复济组和MEBT/MEBO组,每组各20只。模型组、 MEBT/MEBO组和贝复济组大鼠参照糖尿病模型制备法,采用腹腔注射60 mg/kg链脲佐菌素,建立糖尿病动物模型;空白对照组大鼠则腹腔注射等量柠檬酸-柠檬酸钠缓冲液。分别于干预治疗后第5、14天各取相同部位创面组织,采用Western blot法检测TGF-β1、Smad...

Objective:To investigate the effects of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)on the expressions of transforming growth factor-β1(TGF-β1),Smad3 and phosphorylation Smad(P-smad3)and morphological structure in diabetic foot wound of rat. Methods:Eighty healthy male SD rats were randomly divided into blank control group,treated model group, recombinant bovine basic fibroblast growth factor(rb-bFGF)group and MEBT/MEBO group,each group had 20 rats. Rats in treated model group,rb-bFGF group and MEBT/MEBO group were enroll...