期刊论文

中文

湖南中医药大学学报

1674-070X

2014

5

9-13

10.3969/j.issn.1674-070X.2014.05.003.009.05

81202632，81373702:国家自然科学基金 20124323120003:高等学校博士学科点专项科研基金 2011FJ2013:湖南省科技厅重点项目 13JJ3097:湖南省自然科学基金 12B097:湖南省教育厅科学研究项目

本校为第一机构

目的：探讨丹龙醒脑方对大鼠脑缺血损伤后海马CA1区巢蛋白（Nestin）和胶质纤维酸性蛋白（glial fib-rillary acidic protein，GFAP）表达的影响。方法将48只大鼠随机分为假手术组、模型组、依达拉奉组、丹龙醒脑方组。采用大脑中动脉线栓法制备大脑中动脉闭塞再灌注（MCAO）模型，对大鼠进行神经功能缺损评分，采用HE染色观察海马CA1区细胞的形态学改变，采用免疫组化法检测海马CA1区Nestin 蛋白和GFAP蛋白的表达。结果与假手术组比较，模型组治疗3 d后和7 d后的神经功能缺损评分明显升高,差异有统计学意义（P〈0.05）；与模型组比较，依达拉奉组和丹龙醒脑方组治疗3 d后和7 d后的神经功能缺损...

Objective Explore the influence of Danlongxingnao recipe on expression of Nestin protein and GFAP protein in rats CA1 area after focal cerebral ischem ia/reperfusion. Methods The 48 rats w ere randomly divided into sham-operation group, modelling group, Danlongxingnao recipe group and edaravone group.T he m odle of cerebral ischem ia reperfusion (I/R )was induced by middle cerebral artery occlusion (MCAO) and recanalization. Neurological sym ptom score were evaluated. HE staining of the cells was used to observe the morphology changes in hippocam pus CA1 area. Nestin protein and GFAP Protein w...