期刊论文

Content Determination of Gallic Acid, Corilagin and Ellagic Acid in Aqueous Extract from Sanguo Decoction by HPLC

中文

中国中医药信息杂志

1005-5304

2017

24

9

76-79

10.3969/j.issn.1005-5304.2017.09.019

本校为其他机构

目的建立HPLC同时测定三果汤水提物中没食子酸、柯里拉京及鞣花酸含量的方法。方法采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,检测波长为260 nm,柱温30 ℃,流速为1.0 mL/min。结果没食子酸、柯里拉京及鞣花酸3种成分有较好的基线分离,且峰形较好,分别在0.174~2.61 μg、0.04~0.60 μg、0.052~0.78 μg范围内线性关系良好;平均加样回收率分别为99.98%、99.81%、100.12%,RSD均小于2.0%。结论本方法快速、准确、重复性好,可为三果汤水提物的质量控制提供依据。

Objective To establish an HPLC method for the simultaneous determination of contents of gallic acid, corilagin and ellagic acid in aqueous extract from Sanguo Decoction. Methods The analysis was carried out on an Agilent C18 column (4.6 mm × 250 mm, 5 μm), and the mobile phase was composed of acetonitrile and 0.1% phosphoric acid aqueous with gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL/min at column temperature of 30 ℃. Results Gallic acid, corilagin and ellagic acid were completely separated and the peak ...