期刊论文

Detection of Enterococci Resistance and Vancomycin Resistant Gene

中文

中南医学科学杂志

2095-1116

2011

39

4

422-425

10.15972/j.cnki.43-1509/r.2011.04.013

本校为第一机构

目的 了解肠球菌对六种常用抗生素的耐药情况,同时检测耐万古霉素肠球菌(VRE)的耐万古霉素基因型.方法 用K-B纸片琼脂扩散法检测肠球菌对万古霉素、***拉宁、氨苄西林、氨苄西林/舒巴坦、环丙沙星以及庆大霉素等六种常用抗生素的敏感性.采用合6μg/mL万古霉素的琼脂稀释筛选平板法(ADSP)筛选VRE菌株,并用多重聚合酶链反应(multiplex-PCR)检测VRE的耐万古霉素基因型.结果 100株临床分离肠球菌中以粪肠球菌(86％)和屎肠球菌(11％)为主.除万古霉素和***拉宁外,屎肠球菌对其它几种抗生素的耐药率明显高于粪肠球菌(P＜0.05)；庆大霉素高耐株检出率为54％,其对常用抗生素的耐药率明显高于低耐株(P＜...

Objective To study the resistance of Enterococci to commonly used antibiotics and detect the genotypes of Vancomycin-Resistant Enterococci（VRE） .Motheds Drug resistance of Enterococci to vancomycin,teicoplanin,ampicillin,ampicillin/sulbactam,ciprofloxacin and gentamycin were detected by disc agar diffusion method.VRE were screened by agar dilution sieving plate（ADSP） containing 6 μg/mL of vancomycin,and their genotypes were determined using multiplex PCR.Results Among the 100 isolated strains of Enterococci,E.faecalis（86%） and E.faecium（...