期刊论文

Caveolin-1 combined with cyclophilin A improves cholesterol accumulation in rat vascular smooth muscle cells

中文

中南医学科学杂志

2095-1116

2020

48

3

257-262+268

10.15972/j.cnki.43-1509/r.2020.03.008

国家自然科学基金（编号:61976222）； 广东省医学科学技术研究基金项目（编号:A2018318）； 广东省自然科学基金项目（编号:2016A030313755）；

本校为第一机构

观察caveolin-1和cyclophilin A在血管平滑肌细胞荷脂过程中的表达变化及其对细胞内胆固醇蓄积的影响。实验采用Western-blot、免疫荧光法分别定量,定位检测caveolin-1和cyclophilin A蛋白的表达;油红O染色观察细胞内脂滴的形成情况;高效液相检测细胞内外总胆固醇含量;免疫共沉淀检测caveolin-1和cyclophilin A相互作用。结果显示,随着荷脂时间延长,caveolin-1和cyclophilin A的蛋白表达逐渐减弱;细胞内脂滴形成逐渐增多,细胞内总胆固醇含量不断增加;caveolin-1和cyclophilin A在大鼠血管平滑肌细胞中相互结合,并且cyclophilin A活性抑制后,与caveolin-1结合能力减低,而细胞内胆固醇含量增加。这...

To observe the expression changes of caveolin-1 and cyclophilin A in vascular smooth muscle cells during lipid storage and the effect on intracellular cholesterol accumulation.The protein expression of caveolin-1 and cyclophilin A was detected by Western blotting and immunofluorescence.The formation of lipid droplets in cells was observed by oil red O staining.The total cholesterol content inside and outside the cells was analyzed by HPLC.Co-precipitation was used to detect the interaction between caveolin-1 and cyclophilin A.The results showed that the protein expression of caveolin-1 and cyc...