期刊论文

中文

湖南中医药大学学报

1674-070X

2017

37

5

514-518

10.3969/j.issn.1674-070X.2017.05.014

13A067:湖南省教育厅重点项目 201443:湖南省中医药科研重点项目

本校为第一机构

目的 观察咳喘穴位敷贴对慢性支气管哮喘大鼠肺组织JAK1、STAT6表达的影响.方法 将40只SD大鼠随机分为对照组、哮喘模型组、咳喘穴位敷贴组、地塞米松组,每组10只.除对照组外,其余各组采用卵清蛋白致敏并激发的方法 制备慢性支气管哮喘大鼠模型.造模成功后,咳喘穴位敷贴组大鼠相应穴位贴敷咳喘穴位敷贴进行干预治疗,地塞米松组大鼠腹腔注射地塞米松磷酸钠注射液0.5 mg/(kg·d).治疗14 d后取大鼠肺组织,HE染色观察肺组织形态学变化,免疫组织化学、Real time PCR检测肺组织JAK1和STAT6蛋白及mRNA的表达水平.结果 与对照组相比,哮喘模型组大鼠肺组织可见大量炎性细胞浸润,气道内有大量分泌物,肺泡结...

Objective To observe the influence of Kechuan acupoint application therapy on the expression of JAK1, STAT6 in the lung tissue of asthmatic rats. Methods The 40 male SD rats were randomly divided into control group, asth-ma model group, acupoint application group and dexamethasone group, 10 rats in each group. Except the control group, bronchial asthma model rats in other groups were sensitized and stimulated by ovalbumin. After succesfully building the asth-ma model rats, was established, the rats were treated by Kechuan acupoint application. ...