期刊论文

Protective Effect of Breviscapine on Tert-Butyl Hydroperoxide-induced Primary Retinal Ganglion Cell Apoptosis

中文

中华眼视光学与视觉科学杂志

1674-845X

2018

20

8

454-460

10.3760/cma.j.issn.1674-845X.2018.08.002

湖南省教育厅科学研究项目(17C1221) 湖南省研究生创新基金资助项目(CX2016B377、CX2017B434、CX2017B426)。

本校为第一机构

目的:探讨灯盏花素(BVP)对视神经节细胞的保护作用.方法:实验研究.培养大鼠原代视网膜神经节细胞(PRGCs),采用0、50、1 00、200 μmol/L叔丁基过氧化氢(tBHP)诱导细胞氧化损伤建立细胞模型(最后选择100 μmol/L建立模型),给予不同浓度(0、10、20、50 μmol/L)BVP处理后(最终采用20 μmol/L用于后续研究),检测神经节细胞的改变,免疫印迹法检测细胞凋亡相关因子的改变.采用t检验进行数据处理.结果:与空白组对照相比,经tBHP处理的PRGCs凋亡明显增加,凋亡相关蛋白Bcl-2 (P＜0.05)和神经标志蛋白Synaptophysin (P＜0.01)的表达明显减少.与模型组相比,各治疗组PRGCs成活率明显增加,细胞凋亡减少,Bcl-2...

Objective: To observe and explore the protective effects of breviscapine (BVP) on tert-butyl hydroperoxide (tBHP)-induced primary retinal ganglion cell (PRGC) apoptosis. Methods: In this experimental study, PRGCs were isolated by immunopanning from retinas of 1-day-old mice and maintained in culture for 3 days. To induce apoptosis, the PRGCs were then exposed to 0, 50, 100, or 200 μmol/L tBHP (100 μmol/L was chosen to establish models) in the absence or presence of 0,10,20, or 50 μmol/L BVP (20 μmol/L was chosen for follow experiments). The...