期刊论文

英文

Evidence-Based Complementary and Alternative Medicine

1741-427X

2021

2021

10.1155/2021/8087183

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81874406]; Open Fund Project of Integrated Chinese and Western Medicine (First-Class Discipline) from the Hunan University of Chinese Medicine [2019ZXYJH10]; Research Fund Project of the Hunan University of Chinese Medicine [2020XJJJ015]

本校为第一机构

To explore the main active components and effects of cell cycle regulation mechanism of Astragali radix (AR) and Angelicae sinensis radix (ASR) on oxidative damage in vascular endothelial cells, a model of oxidative damage in human umbilical vein endothelial cells (HUVECs) induced by oxidized low-density lipoprotein (ox-LDL) treatment was developed. Based on the “knock-out/knock-in” model of the target component, cell viability, intracellular reactive oxygen species (ROS), and lactate dehydrogenase (LDH) leakage were assessed by Cell Counting Kit-8 assay, fluorescent probe 2,7-di...