期刊论文

He, F.-Y.

中文

中国药学杂志

1001-2494

2005

40

21

1647-1649

10.3321/j.issn:1001-2494.2005.21.015

国家自然科学基金（30472199）； 国家中医药管理局中医药科研基金（02-03ZP41）；

本校为第一机构

目的对大鼠血浆补阳还五汤中黄芪甲苷的含量进行了测定,并进行了药动学研究.方法采用反相高效液相色谱法测定,其条件为色谱柱:C18柱(4.6 mm×250 mm,5 μm);检测波长:203nm;流动相:乙腈-水(35:65),流速:1 mL·min-1;柱压:(15.17±0.4137)MPa;温度:45℃.药动学参数采用3P87软件处理.结果血浆样品中黄芪甲苷线性范围为2.4～12μg,r=0.999 6,回收率为100.2%,RSD为1.100%.黄芪甲苷药动学参数:复方:t1/2α为1.392 h;t1/2β为14.24h;K12为0.162 4 h-1;K21为0.304 5 h-1;K10为0.079 58 h-1;总苷:t1/2α为2.641 h;t1/2β为37.12 h;K12为0.1283 h-1;K21为0.1071 h-1;K10为0.045 74h-1.结论该法快速、简便...

OBJECTIVE: To develop a HPLC method for the determination of astragaloside IV in rat plasma. And to investigate astragaloside IV pharmacokinetics in rat. METHODS: The separation was performed on a C,8 column (4.6 mm ×250 mm, 5 μm). The detection wavelength was at 203 nm. A solution of acetonitrile-water(35:65) was used as the mobile phase with the flow rate of 1 mL&middotmin-1. The temperature was 45 °C. The pharmacokinetic parameters were evaluated with 3P87. RESULTS: The calibration curve was linear in the range from 2.4 to 12 μg (r = 0.999 6). The average recovery was (100.2...