期刊论文

Yan, M.

中文

中国药学杂志

1001-2494

2015

50

3

263-268

10.11669/cpj.2015.03.017

国家自然科学基金资助项目（81202985 81473411） 中央高校基本科研业务管理费中南大学青年教师助推项目（2012QNZT151）

本校为其他机构

目的建立测定大鼠血浆中16种胆汁酸浓度的LC-MS/MS法。方法鼠血浆样品经过甲醇沉淀蛋白后,上清液挥干,流动相复溶后,用Xtimate~(TM) C_(18)柱分离,以甲醇-醋酸铵及甲酸水溶液为流动相进行梯度洗脱,流速0. 25 mL ? min~(-1),进样5 μL 分析。结果16种胆汁酸在0.005 6 ~3. 800 0 μmol ? L~(-1)内,线性关系良好,定量下限浓度为0. 005 6 μmol ? L~(-1),低、中、高浓度的萃取回收率在70.3%~107.2%之间,日内、日间RSD均小于12.9%。结论该方法准确可靠,灵敏度高,处理简便,适用于血浆中胆汁酸浓度的测定,为临床血浆样品中胆汁酸含量测定提供参考。

OBJECTIVE: To establish an LC-MS/MS method for determination of the concentrations of 16 bile acids in rat plasma. METHODS: The plasma protein was precipitated with methanol and then the supernatant was dried. Liquid chromatographic separation was performed on a Xtimate™ C18 column with gradient elution of mobile phase consisting of methanol-ammonium acetate and formic acid buffer, the flow rate was 0.25 mL · min-1, and the injection volume was 5 μL. RESULTS: The calibration curves were linear in the concentration range of 0.0056-3.8000 μmo...