期刊论文

Wu, P.

中文

中国药学杂志

1001-2494

2017

52

7

548-552

10.11669/cpj.2017.07.007

湖南省教育厅重点项目（14A109）资助项目 湖南省发改委项目（[2013]1199）资助项目 湖南省科技厅科研条件创新专项（2012TT2012）资助项目 湖南省研究生省级科研创新项目（CX20158336）资助项目

本校为第一且通讯机构

目的研究经分段醇沉所得铁皮石斛多糖(DOP)对巨噬细胞(RAW264.7)的免疫调节作用。方法采用分步醇沉法得到不同相对分子质量的铁皮石斛多糖,四唑盐比色法(MTT)检测DOP对脂多糖(LPS)诱导巨噬细胞的保护作用,中性红法检测巨噬细胞的吞噬活性,酶联免疫吸附实验法(Elisa)检测培养液中肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、转化生长因子- β1(TGF-β1)的含量,格里斯试剂比色法(Griess)检测一氧化氮(NO)的释放水平,流式细胞仪检测细胞的极化分型。结果醇浓度为75%时,醇沉的多糖得率最大;DOP能促进RAW264.7细胞增殖,增强巨噬细胞的吞噬活性,降低巨噬细胞TNF-α、IL-6、NO的释放水平,促进细胞分泌TGF-...

OBJECTIVE: To study the immunoregulatory effect of Dendrobium officinale polysaccharide (DOP) obtained by step ethanol precipitation on macrophages and explore its action mechanisms. METHODS: Step ethanol precipitation method was used to get DOPs of different molecular weights, MTT method was used to detect the protective effect of DOP on macrophages induced by LPS, neutral red method was used to detect the phagocytosis activity of macrophages, ELISA method was used to detect the contents of TNF-α, IL-6, and TGF-β1, and Griess method was used...