期刊论文

Mechanism Study of Supernatants of Alcohol Extracting-Water Precipitation of Polygonatum odoratum on Hyperthyroidism-yin Deficiency Rats Based on GC-MS Metabonomics

Xia, B.-H.

中文

中国药学杂志

1001-2494

2017

52

23

2092-2097

10.11669/cpj.2017.23.009

(2015468002-3):国家中医药管理局中医药行业科研专项资助 (0901018009):湖南省重点实验室科研资助项目 (99820001-194):湖南中医药大学校级课题资助项目

本校为第一且通讯机构

目的初步探讨玉竹水提醇沉上清液(POS)治疗甲亢阴虚的作用机制。方法将SD大鼠随机分为正常组,模型组,玉竹组(400 mg · kg~(-1));通过连续1周腹腔注射T3溶液(500 μg · kg~(-1))建立大鼠甲亢阴虚模型,测定血浆中cAMP、cGMP、血清中T_3、T_4等指标;采用GC-MS技术分析大鼠血清代谢组图谱,运用软件Simca-P12.0进行多元统计分析。结果POS可以显著降低模型动物的T_3、T_4、cAMP、cAMP/cGMP含量,升高cGMP含量,对大鼠阴虚具有显著的改善作用。主成分分析和偏最小二乘法判别分析得分图显示正常组、模型组和玉竹组的代谢轮廓有明显差别,并呈现趋势性变化,其中POS干预的阴虚大鼠整体代谢轮廓趋向正常水平...

OBJECTIVE: To evaluate the effect of supernatants of alcohol extracting-water precipitation of Polygonatum odoratum(POS) on hyperthyroidism-yin deficiency rats. METHODS: SD rats were randomly divided into control, model and POS groups(400 mg·kg-1). The hyperthyroidism-yin deficiency rats were treated by intraperitoneal injection of T3(500 μg·kg-1) for a week. The content of T3, T4 in serum and cAMP, cGMP in plasma was determined. A GC-MS based metabolic profiling method was employed to analyze the distinctive metabolic patterns of serum in c...