期刊论文

Screening of the Appropriate Proportion on Anti-Breast Cancer Activity of Prunellae spica Combined with Taraxaci herba and Its Anti-Tumor Research in Vivo

Lin, L.-M.

中文

中国药学杂志

1001-2494

2018

53

10

776-782

10.11669/cpj.2018.10.004

国家自然科学基金项目资助（81503041） 国家科技部公益性行业专项资助（201507002） 湖南中医药大学校级课题资助（2016） 湖南省自然基金资助（2017JJ1023,2017JJ4045） 中国.卢森堡中医药合作项目资助（国家中医药管理局函[2015]105号）

本校为第一且通讯机构

目的基于联合指数法( combination index,CI)体外筛选夏枯草( PV)配伍蒲公英( TM) ( PV + TM)抗乳腺癌最佳配伍比例并探讨其最佳比例对4T1荷瘤小鼠肿瘤细胞增殖和凋亡的影响。方法四甲基偶氮唑蓝( MTT)法检测PV + TM联合应用抗乳腺癌细胞增殖活性; CI评价PV + TM联合作用效价,当CI < 1时认为两药具有协同效应;通过皮下注射4T1细胞建立移植瘤小鼠模型,持续给药25 d,评价PV + TM体内抗肿瘤活性;小鼠体重和肿瘤体积每3 d测量1次;摘取肿瘤组织,HE染色检测病理结构变化,TUNEL染色法检测药物的促凋亡活性。结果与单独用药相比,PV + TM显著提高了对MCF-7和MDAMB- 231细胞的抗增殖活性,并且在夏枯草与蒲...

OBJECTIVE: To screen optimal ratio of Prunella vulgaris(PV) and Taraxacum mongolicum(TM) as a synergetic combination for anti-breast cancer treatment in vitro based on combination index(CI) and to investigate the effects of PV and TM on cell proliferation and apoptosis under the optimized ratio in tumor-bearing(4T1) female BALB/c mice in vivo. METHODS: Cell proliferation was assayed by MTT method. The dose-effect of PV combined with TM was evaluated by CI method, The combination has a synergistic effect when the CI value is less than 1. Breast cancer model was established by subcutaneous injec...