期刊论文

Reversal Effect and Mechanism of 15-PGHD Induction Drugs on the Multidrug Resistance of Human Breast Cancer Cell Line MCF-7 /ADR

Liu, S.-K.

中文

中国药学杂志

1001-2494

2018

53

16

1352-1358

10.11669/cpj.2018.16.004

本校为第一机构

目的探讨15-羟前列腺素脱氢酶( 15-PGDH)在人乳腺癌多药耐药细胞中的表达,并观察15-PGDH诱导药物对MCF-7 / ADR细胞耐药性的影响.方法采用逆转录-聚合酶链反应( RT-PCR) 、Western blot检测MCF-7和MCF-7 /ADR细胞中15- PGDH、环氧合酶-2( COX-2) mRNA和蛋白表达; ELISA检测细胞上清液中PGE_2含量;四甲基偶氮唑蓝( MTT)法测定15-PGDH诱导药物(吲哚美辛、布洛芬、吡格列酮、地塞米松)对乳腺癌细胞的增殖抑制作用及对多柔比星( adriamycin,ADM)敏感性的影响; Hochest 33258检测细胞凋亡.结果与MCF-7相比,MCF-7 /ADR细胞中15-PGDH表达水平明显降低,COX-2表达明显增加,细胞上清液中PGE_2水平显著增加...

OBJECTIVE: To examine the expression of 15-hydroxyprostaglandin dehydrogenase(15-PGDH) in human multidrug-resistant breast cancer line MCF-7/ADR and to explore the reversal effect and mechanism of 15-PGHD induction drugs on MCF-7/ADR cells. METHODS: The RT-PCR and Western blot were used to detect 15-PGDH, COX-2 mRNA and protein expression in MCF-7 and MCF-7/ADR cells. PGE2 levels in supernatant of cells were determined by ELISA assay. Anti-proliferation effect and chemotherapy sensitivity to ADM of 15-PGDH induction drugs (indomethacin, ibuprofen and pioglitazone, dexamethasone) on breast canc...