期刊论文

Peng, J.-P.

中文

世界华人消化杂志

1009-3079

2008

16

31

3556-3560

10.11569/wcjd.v16.i31.3556

国家中医药管理局中医药科学技术研究专项资助项目,No.06-07JB17作者贡献分布：此课题由彭建平与孙克伟设计：研究过程由彭建平,伍玉南及何芳操作完成：研究所用新试剂及分析工具由孙克伟提供：数据分析由彭建平与孙克伟完成：本论文写作由彭建平完成.

本校为第一且通讯机构

目的:探讨乙型肝炎患者外周血DCs HBV DNA载量与DCs功能的关系.方法:采集20例慢性乙型肝炎患者和10例健康人的抗凝外周静脉血,分离外周血单个核细胞(PBMCs),IL-4和GM-CSF的作用下培养使DCs增殖、成熟,以流式细胞技术分别检测DCs表面HLA-DR、CD-1α、CD80及CD86的表达:ELISA检测DCs培养上清液IL-12的水平,实时荧光定量PCR法检测DCs HBV DNA的载量,同种异体混合淋巴细胞反应检测DCs刺激同种异体T细胞增殖能力.结果:慢性乙型肝炎患者外周血DCs内亦可检测至HBv DNA,患者DCs表面HLA-DR、CD-1α、CD80及CD86的表达水平、DCs分泌IL-12水平及DCs刺激同种异体T细胞增殖能力均显著低于健康对照组,其中DCs ...

AIM: To investigate the HBV DNA load in monocyte-derived dendritic cells (DCs) from patients with chronic hepatitis B, and to explore the reason for decrease of DC function. METHODS: Twenty patients with chronic hepatitis B and 10 healthy controls were included in this study. We incubated and induced DC subsets differentiated from peripheral blood mononuclear cells (PBMCs). The expression levels of HLA-DR, CD-1α, CD80 and CD86 were detected by flow cytometry, and the levels of interleukin-12 (IL-12) in supernatants were measured by enzyme-link...