期刊论文

Effect of acupuncture with varied intensity on NMDA and SP expression in spinal dorsal horn in rats with gastric distension-induced pain

Yi, S.-X.

中文

世界华人消化杂志

1009-3079

2009

17

13

1339-1345

10.3969/j.issn.1009-3079.2009.13.012

国家重大基础研究(973)基金资助项目 教授 410007 湖南中医药大学东塘校区. yishouxiang@yahoo.com.cn 电话: 0731-5381161.通讯 No. 2005-CB523306-9 湖南省自然科学基金资助项目 No. 08C632易受乡 No. 06JJ50049 湖南省教育厅基金资助项目 湖南省长沙市韶山中路113号

本校为第一且通讯机构

目的:探讨毫针不同刺激强度针刺足三里穴后对胃扩张大鼠的拟痛行为学、脊髓背角N-甲基-D-天冬氨酸(NMDA)受体表达和P物质(SP)含量的影响.方法:50只大鼠按完全随机法分成5组, 分别为空白组、假手术组、模型组、轻手法组和重手法组. 采用胃内植入气囊并充气压40mmHg造成胃扩张疼痛模型, 轻手法和重手法针刺足三里穴为施治因素, 以拟痛行为学评分标准观察其拟痛行为学, 用免疫组织化学法检测脊髓背角中NMDA受体表达和SP含量.结果:与假手术组比较, 胃扩张造模后, 大鼠拟痛行为学评分和脊髓背角内NMDA受体均明显增加( P

AIM： To explore the effect of varied intensity acupuncture on pain-ethology scoring and on expression of n-methyl-d-aspartate receptor （NMDA receptor） and substance P （SP） in spinal dorsal horn quasi-pain in rats with pain induced by gastric distension. METHODS： Fifty healthy male Sprague Dawleyrats were randomly assigned to blank, sham operation, model, acupuncture of lighter stimulus intensity at ＂Zusanli＂ point and acupuncture of heavier stimulus intensity at ＂Zusanli＂ point groups. Gastric distention model was established by apply...