期刊论文

Tan, D.-M.

中文

世界华人消化杂志

1009-3079

2009

17

24

2444-2450

10.3969/j.issn.1009-3079.2009.24.002

湖南省科技厅基金资助项目,No.06SK3003~~；

本校为其他机构

目的: 探讨TGF-β1、TGF-β1R siRNA联合应用对小鼠急性肝损伤TGF-β/Smad信号传导通路的干预作用.方法: 清洁级健康大鼠36只分为2组: 正常对照组(n=6, 注射生理盐水)与实验组(n =30).实验组动物在实验第1天和第5天腹腔注射CCl4花生油溶液6 mL/kg, 造成急性肝损伤并启动TGF-β/Sma d信号传导通路.实验分为TGF-β1 shRNA干预组(T);TGF-β1siRNA+TβRⅠshRNA干预组(T+R1);TGF-β1shRNA+TβRⅡ siRNA干预组(T+R2);TGF-β1siRNA+TβRⅠshRNA+TβRⅡ shRNA干预组(T+R1+R2)和模型对照组(M).观察各实验组血清ALT、AST、HA;肝组织学RT-PCR和免疫组织化学检测肝组织中α-SMA、COL-1、COL-3、TGF-β1、Sma...

AIM: To investigate the inhibitory effect of combined use of smal l inter fer ing RNAs (siRNAs) specific for transforming growth factor beta 1 (TGF-β1) and transforming growth factor beta 1 receptors (TGF-β1 RI and TGF-β1 RII) on the TGF-β/Smad signaling pathway in rats with acute liver injury. METHODS: Thirty rats were given two intraperitoneal injections of carbon tetrachloride (CCl 4 ; 6 mL/kg) to induce acute liver injury and initiate the TGF-β/Smad signaling pathway. The rats were then divided into five groups: TGF-β1 siRNA intervent...