期刊论文

Yi, S.-X.

中文

世界华人消化杂志

1009-3079

2010

18

31

3287-3293

国家自然科学基金资助项目,No.30772707； 教育部博士点科学基金资助项目,No.20070541003~~；

本校为第一且通讯机构

目的:探讨艾灸血浆对乙醇损伤的离体人胃黏膜上皮细胞(GES-1)的保护作用.方法:将离体胃黏膜上皮细胞(GES-1)分为4组,即空白组、乙醇损伤模型组、艾灸穴位血浆组和艾灸非穴位血浆组. 艾灸人体足三里、中脘、关元穴及非穴对照点前后提取并制备艾灸前后的血浆. 以含8%乙醇培养液作用于后3组胃黏膜上皮细胞,建立胃黏膜上皮细胞损伤模型. 各组分别加入预先提取的灸前血浆、艾灸穴位血浆和艾灸非穴位血浆. 采用MTT法检测细胞抑制率,比色法检测细胞培养上清液中液乳酸脱氢酶(lactate dehydrogenase,LDH)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)的含量,流式细胞技术检...

AIM: To investigate whether plasma of individuals undergoing moxibustion has protective effects against ethanol-induced injury to human gastric epithelial cells (GES-1). METHODS: Isolated GES-1 cells were divided into four groups: control group, ethanol injury group, test plasma group (plasma from individuals undergoing moxibustion at specific points), and control plasma group (plasma from individuals undergoing moxibustion at non-specific points). Before and after moxibustion at Zusanli, Zhongwan, Guanyuan or non-specific points, blood plasma ...