期刊论文

Rroteomics Effect of Treating Atherosclerosis Rat by Yinchenwulin Powder

中文

浙江中医药大学学报

1005-5509

2005

29

1

41-44

10.3969/j.issn.1005-5509.2005.01.025

本校为其他机构

目的:探讨茵陈五苓散对动脉粥样硬化(AS)大鼠作用的分子机制,方法:20只雄性Wistar大鼠采用高脂饲料喂饲法复制大鼠AS模型,造模1月后抽样检测大鼠主动脉,以发现动脉粥样硬化斑块为造模成功指标.茵陈五苓散治疗1个月后分别用固相pH梯度双向凝胶电泳分离茵陈五苓散组和模型组的AS大鼠主动脉组织总蛋白质,银染显色,PDQuest 2-de软件分析差异蛋白质点.结果: 双向电泳图象分析茵陈五苓散组图象的平均蛋白质数为1077±68,模型组的平均蛋白质数为1106±84;差异蛋白质有556个,其中有196个表达增强,有360蛋白质表达下降.结论: 茵陈五苓散的抗AS作用与这些差异表达蛋白质有关,这些差异蛋白质有待质谱分析鉴...

Objective: Proteomics is currently in a phase of technological development and establishment, demonstrating the capacity for high throughput, is a major challenge. It is used in life science research, particulay in the mechanism of tumrogenesis. Yinchenwulin powder, which was extracted from Chinese herbs, is used to treat the patients with atherosclerosis. We investigated the molecular mechanism of Yinchenwulin Powder on the atherosclerotic rats in this paper. Methods: The AS model wistar rats made by feeding with high grease food and AS enplaques were observed in the aorta after one month of ...