期刊论文

Inhibition of the hypoxic activation of hypoxia inducible factor-1α by docetaxel in uterine cervical carcinoma

中文

现代医药卫生

1009-5519

2008

24

8

1109-1111

10.3969/j.issn.1009-5519.2008.08.002

本校为第一机构

目的：探讨多西紫杉醇对宫颈癌细胞低氧诱导因子-1α基因表达的影响。方法：Hela细胞在正常条件下（氧气浓度20％）培养后，对照组在正常条件下继续培养24小时，试验组转入缺氧条件下培养24小时，氧气浓度分别为1％。采用反转录聚合酶链式反应（RT-PCR）法检测HIF-1αmRNA表达变化，蛋白质免疫印迹法测定HIF-1α蛋白水平表达变化，MTT法检测肿瘤细胞抑制率。结果：多西紫杉醇可抑制宫颈癌细胞生长，正常对照组有一定水平的HIF-1αmRNA表达，但几乎检测不到HIF-1α蛋白表达，低氧条件下HIF-1αmRNA表达维持在高水平，但HIF-1α蛋白则明显升高（t=2．471-5．474，P〈0．01）；加入5×10^-6mmol／L...

Objective ：To investigate the effect of docetaxel on the hypoxic activation of hypoxia inducible factor（HIF）-1α in uterine cervical carcinoma.Methods：Hela cells were cultured under normoxic condition.The control group was kept under normoxic condition for 24h ,the experimental groups were kept under hypoxic conditions（1% O2） for 24h with or without docetaxel. Growth rate was measured by MMT assay. The HIF-1 α expression was measured by RT-PCR and immunoblot.Results：Docetaxel inhibited the growth of Hela cells. Under normoxic condition,...