期刊论文

中文

现代医药卫生

1009-5519

2018

34

24

3749-3752

10.3969/j.issn.1009-5519.2018.24.002

81473615:国家自然科学基金 2015JJ2017:湖南省科技厅自然基金项目

本校为第一机构

目的 探讨动脉粥样硬化Toll样受体4基因敲除(TLR4-/-)小鼠模型卷曲蛋白(FZD)变化特征及护心康对动脉粥样硬化的干预作用.方法 SPF级TLR4-/-小鼠70只,随机选择12只纳入正常对照组,予以普通饲料喂养;其余58只予以高脂饲料造模,喂养13周后随机选择5只解剖观察胸主动脉,光镜下发现粥样斑块为造模成功.造模成功动物随机分为模型组、护心康组、阿托伐他汀组,Wnt抑制因子(WIF)组,每组12只,分别予以相应处理.处理8周后处死小鼠,心脏穿刺采血检测血脂水平;取胸主动脉,光镜下观察动脉粥样硬化斑块形成情况;免疫组织化学法检测斑块中FZD蛋白的表达.结果 模型组胆固醇、三酰甘油、低密度脂蛋白和高密度脂蛋白...

Objective To investigate the changes of Frizzled protein(FZD)and the intervention of Huxinkang in Toll like receptor 4 gene knockedout(TLR4^-/-)atherosclerosis mice model.Methods 70 SPF TLR4^-/-mice were fed,among which 12 mice were randomly selected as control group and fed with normal diet.The remaining 58 mice were modeled with high-fat diet.After 13 weeks of feeding,5 mice were randomly selected from modeled mice to observe the thoracic aorta,and atheroma plaque found under light microscopy indicated successful modeling.The successful model animals were randomly divided into model group,Hu...