期刊论文

Rapid Detection of Staphylococcus aureus by Using Graphene Oxide-Based Biosensor Based on Enzyme-Aided Signal Amplification

中文

分析科学学报

1006-6144

2019

35

1

25-30

10.13526/j.issn.1006-6144.2019.01.005

湖南省自然科学基金(No.2016JJ3098) 湖南省教育厅重点项目(No.2016A027).

本校为其他机构

本研究以设计的与16S rRNA互补的单链DNA (ssDNA)探针作为捕捉探针,羧基荧光素(FAM)标记的探针1、探针2作为信号探针,将两者组装形成两个FAM标记的探针(TFP),并以氧化石墨烯(GO)作为猝灭剂形成TFP/GO复合物.当无靶标时,TFP吸附到GO表面,FAM的荧光被猝灭,当加入靶标后,TFP从GO上解离下来,荧光恢复,因此可以通过荧光值定量检测靶标.此外,在体系中加入脱氧核糖核酸酶Ⅰ (DNase Ⅰ),TFP被降解从而释放出靶标RNA用于下一轮反应同时产生大量的FAM,可以极大地提高该传感器的灵敏度及特异性.结果表明该方法对靶标RNA的检测限可达0.3 nmol/L,且在1～60 nmol/L范围内呈良好的线性关系(R2=0.9984);对靶标菌...

In this study,the ssDNA used as capture probe that is complementary to 16SrRNA and the carboxyfluorescein(FAM)-labelled probe 1 and probe 2 used as signal DNA probe is designed.The two FAM-labeled probe(TFP)are assembled by the two probe,meanwhile,TFP/graphene oxide(GO)is formed by using GO as the quencher.In the absence of target,TFP is adsorbed onto the surface of GO, resulting in the fluorescence quenching of the dye.When the target is introduced,the fluorescence is restored due to the probe being dissociated from GO.The target quantum is ac...