Graphene Oxide-Based Fluorometric Determination of the eta Gene in Pseudomonas aeruginosa Using Nicking Enzyme-Mediated Cyclic Signal Amplification

首页 > 成果 > 详情

认领

导出

Link by DOI

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

He, Qizhi;Ma, Shuheng;Wang, Jingya;Chen, Keke;Dong, Jun;...

通讯作者：

Danna Chen<&wdkj&>Yi Ning

作者机构：

[Chen, Keke; Zhou, Ji; Dong, Jun; He, Qizhi; Chen, Danna; Ma, Shuheng; Wang, Jingya] Changsha Med Univ, Academician Workstn, Changsha 410219, Hunan, Peoples R China.

[Ning, Yi] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Changsha 410208, Hunan, Peoples R China.

[Zhou, Ji; He, Qizhi; Chen, Danna] Changsha Med Univ, Sch Basic Med Sci, Changsha, Hunan, Peoples R China.

[Dong, Jun; He, Qizhi; Ma, Shuheng] Changsha Med Univ, Discipline Basic Med Applicat, Changsha, Hunan, Peoples R China.

通讯机构：

[Danna Chen] A

[Yi Ning] D

Academician Workstation, Changsha Medical University, Changsha, Hunan, People’s Republic of China<&wdkj&>School of Basic Medical Science, Changsha Medical University, Changsha, Hunan, People’s Republic of China<&wdkj&>Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, People’s Republic of China

语种：

英文

关键词：

Enzyme-assisted recycling amplification;fluorescent assay;graphene oxide;Pseudomonas aeruginosa detection;quenching

期刊：

Analytical Letters

ISSN：

0003-2719

年：

2022

卷：

期：

页码：

1027-1039

DOI：

10.1080/00032719.2021.1980885

基金类别：

We would like to thank National Natural Science Foundation (81803964), Natural Science Foundation of Hunan Province (2019JJ50693, 2020JJ4465, 2018JJ3573), Scientific Research Project Funded by Education Department of Hunan Province (18A493, 18C1156), Hunan Key Laboratory Cultivation Base of Research and Development of Novel Pharmaceutical Preparations (2016TP1029), Excellent Young Innovators of Changsha (kq2009092) and Foundation of Education Bureau of Hunan Province (18A493) for the financial support. We would like to thank Editage for English language editing.

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

A convenient and highly sensitive bioassay employing graphene oxide (GO) as a nanocarrier and nicking enzyme (Nb.BbvC I)-assisted signal amplification was developed to detect Pseudomonas aeruginosa. Furthermore, the assay employed a DNA probe with a capture probe (CP) and a help probe (HP), which were designed to bind the target and signal probe (SP), respectively. In the absence of the target, the SP did not bind to the HP due to steric effects. Consequently, the fluorescence of carboxyfluorescein (FAM) was quenched by the black hole quenching...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

Graphene Oxide-Based Fluorometric Determination of the eta Gene in Pseudomonas aeruginosa Using Nicking Enzyme-Mediated Cyclic Signal Amplification

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问