期刊论文

英文

Talanta

0039-9140

2023

259

124549

10.1016/j.talanta.2023.124549

National Natural Science Foundation [81803964, 82074250]; Xinglin talent cultivation program; Natural Science Foundation of Hunan Province [2020JJ4465, 2020JJ4063]; Key Project of the Department of Education of Hunan Province [21A0237]; Western medicine in Hunan Province [2021ZXYJH14]; Basic medicine construction project of Hunan University of traditional Chinese Medicine [6]; Hunan provincial key laboratory of integrated traditional Chinese and Western medicine [2019TP1044]

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We describe a graphene oxide (GO)-based bioassay for the fluorometric determination of norA gene transcription (mRNA) in methicillin-resistant Staphylococcus aureus (MRSA). This approach is based on Nb.BbvCI-assisted target recycling (NATR) and T7 exonuclease (T7 Exo)-triggered cascade dual-recycling signal amplification (TTCDRSA). The system included GO, a capture probe (CP), an assistant probe (AP), two carboxyfluorescein (FAM)-labeled hairpins (HP1 and HP2), endonuclease Nb.BbvcI, and exonuclease T7. In the presence of a target, AP, together with the target RNA, can hybridise with CP via pa...