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Identification of phage display peptides with affinity for the tegument of Schistosoma japonicum schistosomula

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成果类型:
期刊论文
作者:
Liu, Yan(刘碧源);Brindley, Paul J.;Zeng, Qingren;Li, Yuesheng;Zhou, Jun;...
通讯作者:
Zeng, QR
作者机构:
[Yang, Shenghui; Liu, Yan; Zhang, Zuping; Cai, Liting; Zeng, Qingren; Chen, Yuxiao; Li, Yuesheng] Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha 410013, Hunan, Peoples R China.
[Brindley, Paul J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol & Trop Med, Washington, DC 20037 USA.
[McManus, Donald P.; Li, Yuesheng] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld 4006, Australia.
[Liu, Yan] Univ S China, Sch Med, Dept Parasitol, Hengyang 421001, Peoples R China.
[Zhou, Jun] Cent S Univ, Xiangya Hosp 3, Expt Ctr, Changsha 410013, Hunan, Peoples R China.
通讯机构:
[Zeng, QR ] C
Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Tongzipo Rd 172, Changsha 410013, Hunan, Peoples R China.
语种:
英文
关键词:
helminth protein;peptide library;peptide ZL4;polypeptide;rhodamine B;unclassified drug;absorption;article;binding affinity;biotransformation;conjugation;controlled study;developmental stage;host parasite interaction;immunoblotting;immunohistochemistry;in vitro study;in vivo study;integument;nonhuman;parasite identification;peptide synthesis;phage display;priority journal;protein binding;protein isolation;protein localization;real time polymerase chain reaction;Schistosoma japonicum;Schistosomulum;surface property;trematode life cycle stage;Animals;Female;Humans;Kinetics;Mice;Peptide Library;Peptides;Protein Binding;Schistosoma japonicum;Schistosomiasis japonica;Mammalia;Schistosoma;Schistosoma japonicum
期刊:
Molecular and Biochemical Parasitology
ISSN:
0166-6851
年:
2011
卷:
180
期:
2
页码:
86-98
基金类别:
Financial support: This study was supported financially by the Natural Science Foundation of China (Grant No. NSFC:30570952 ), the Hunan Provincial Science Foundation ( 10JJ3010 ), the Foundation of Hunan Provincial Health ( B2009051 ), the Key Projects Fund of Central South University ( 2010QZZD027 ) and the Key Projects Fund of Changsha City ( K0802105-31 ).
机构署名:
本校为其他机构
院系归属:
医学院
摘要:
Peptides, bound to the tegument of live Schistosoma japonicum schistosomula, were differentially screened by phage display in vitro using three rounds of reverse absorption and bio-panning. Three M13 phage peptides were isolated and identified by determination of their recovery rate, immunohistochemical localization, immunoblot analysis, and their anti-schistosomal effects in vivo and in vitro. Of the three, M13 phage peptide ZL4 (MppZL4, YSGLQDSSLRLR, 1.4 kDa, pI 8.8) bound to the tegument of mechanically transformed schistosomula and to other...

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