期刊论文

中文

中华中医药学刊

1673-7717

2009

27

1

57-60

10.13193/j.archtcm.2009.01.59.chengxh.011

基金项目：国家自然科学基金资助项目（30572408） 湖南省自然科学基金资助项目（07JJ604）

本校为第一机构

目的:建立二亚硝基哌嗪（DNP）诱导气虚体质状态大鼠鼻咽黏膜上皮细胞癌变模型,探讨大鼠鼻咽癌变过程中细胞凋亡和增殖的变化及相关性。方法:SD大鼠随机分为模型组Ⅰ、模型组Ⅱ和空白对照组。模型组Ⅰ腋部皮下注射DNP和佛波醇酯（TPA）,模型组Ⅱ进行力竭游泳的同时注射DNP和TPA,空白组同法注射用生理盐水稀释10倍的DMSO。定期分批处死大鼠,取鼻咽组织做病理检测,TUNEL法检测细胞凋亡活性,免疫组织化学SABC法检测PCNA蛋白活性表达。结果:随DNP诱导时间的延长,气虚体质大鼠鼻咽上皮细胞病理变化日趋明显,最终发生恶变。在同一实验时段,模型组Ⅱ鼻咽上皮细胞病变发生率明显高于模型组Ⅰ。在正常鼻咽...

Objective：To investigate on the apoptotisis and proliferating on rats with nasopharyngeal cancerous lesion induced by qi deficiency toxin-contamination pathogenesis. Methods ： Included in this part of study were SD rats, randomly divided into 3 groups, i. e. modeling group Ⅰ （MG1） ,modeling group Ⅱ （MG2）and healthy controlling group（HCG）. The rats in MG1 were sub-cutaneously injected in axillary fossa with N, N′-dinitrosopiperazine （DNP）and 12-O-tetradeeanoylphorbol（TPA） simply,those in MG2 were also injected with DNP and TPA in ...