期刊论文

中文

中华中医药学刊

1673-7717

2009

27

4

724-726

10.13193/j.archtcm.2009.04.53.zhangyj.073

国家自然科学基金资助项目（30672724） 国家教育部博士专项科研基金资助项目（20050541003）

本校为第一机构

目的:为了探讨乙醇灌胃法对SD大鼠和其自身启动的修复机制及相关的信号分子的影响。方法:雄性SD大鼠20只,适应性喂养1周后,禁食24h,取10只用无水乙醇按1mg/kg灌胃造模型,其余10只灌以相同剂量的生理盐水。24h后采用链霉蛋白酶消化法分离胃黏膜细胞,用表面加强激光解析电离飞行时间质谱技术（SELDI-TOF-MS）及WCX2蛋白芯片获得胃黏膜损伤大鼠和对照组大鼠胃黏膜细胞的蛋白质指纹图谱,通过对比分析两组的差异的蛋白质荷比峰,从而寻找出乙醇致胃黏膜损伤的特异相关分子。结果:对照组与模型组比较共有4个蛋白质有显著性差异（P

Objective ：To investigate the effect of the way of intragastric administration of alcohol to SD rats , itself priming repair mechanism and relative signaling molecules. Methods ：20 male SD rats,to adaptability feed a week,then to absolute diet 24 hours,to randomly take 10 SD rats with dehydrated alcohol according to 1mg/kg to intragastric administration for making model,the other 10 SD rats to intragastric administration with physiological saline according to the equal dosage. After 24 hours, the gastric mucosal cells were separated by pronas...