期刊论文

Effects of Simo Decoction on Ghrelin Nos in Cold- Constraint Stressed Mice

中文

中华中医药学刊

1673-7717

2014

32

8

1877-1879

10.13193/j.issn.1673-7717.2014.08.024

国家重点基础研究计划（973计划）项目（2009CB523002）；

本校为第一机构

目的:探讨四磨汤对寒冷束缚应激小鼠脑肠肽NOS的影响。方法:动物随机分组,采用寒冷束缚等多种刺激方法,制备胃肠功能紊乱小鼠模型,分别给予蒸馏水、得舒特和四磨汤口服液治疗;免疫组织化学法观察小鼠脑内NOS的阳性表达,用RT-PCR法检测结肠组织NOS mRNA表达。结果:模型组小鼠NOS阳性表达减少,平均OD值与正常组比较差异有显著性统计学意义（P

Objective：To study the effects of Simo Decoction on expression of NOS in cold - constraint stressed mice. Methods：Gastrointestinal dysfunction model was established by exerting the factors of cold - constraint stimulation. And then they were randomly treated with distilled water, Dieetel and SD. The expressions of NOS in brain tissue were meas- ured by immunohistoehemieal staining. The expressions of NOS in intestine were measured by RT - PCR. Result ： The ex- pressions of NOS in brain tissue of model group was decreased significantly compar...