期刊论文

Effect of Qingguang’an Slow-release Agent to Related mRNA of TGF-β/Smad Signal Transduction Pathway in Fltering Scarring after Glaucoma Filtering Surgery

中文

中华中医药学刊

1673-7717

2019

37

5

1075-1078+1284

10.13193/j.issn.1673-7717.2019.05.013

国家自然科学基金项目(81273807,81403437) 国家中医药管理局中医眼科学重点学科建设项目 教育部博士学科点基金项目(20124323110006) 湖南省自然科学基金重点项目(11JJ2050) 湖南省研究生创新基金重点项目(CX2013A013) 中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018) 中央财政支持地方高校建设项目 湖南省中医五官科学重点学科建设项目 长沙市科技计划项目(kc1704005) 湖南中医药大学中医学国内一流建设学科项目(2018).

本校为第一机构

目的:观察青光安缓释剂对抗青光眼术后滤过道瘢痕化过程中TGF-β/Smad信号传导通路相关mRNA的影响,探讨青光安缓释剂对滤过道瘢痕化的效果及作用机制,并判断其安全性。方法:通过高通量中药筛选体系提取4种青光安有效组份,并制成缓释药膜。将48只实验用兔分为8组,每组6只。分别为:A:空白组;B:模型组;C:MMC对照组;D:有效组份1组;E:有效组份2组;F:有效组份3组;G:有效组份4组;H:安慰剂组。除A组外,其余各组兔眼行常规小梁切除+虹膜根部切除术;C组在术中联合应用MMC;D、E、F、G 4组分别在结膜下植入相对应的青光安缓释药膜;H组植入安慰剂膜片。术后各组进行眼部常规检查。用药4周后处死实验用兔,取术...

Objective: To observe the effect of Qingguang’an slow-release agent to the related mRNA of TGF-β/Smad signal transduction pathway in the filtering scarring after glaucoma filtering surgery. To study the mechanisms of Qingguang’an slow-release agent and preliminarily determine their safety. Method: By using high throughput drug screening system,we screened out 4 effective elements from Qingguang’an. And 4 effective elements were made into sustained-release film. Forty-eight rabbits were randomly divided into 8 groups, 6 rabbits in each group...