期刊论文

Effect of Ursolic Acid on Imbalance of Th17/Treg Ratio in Lymphocytes of CIA Model Rats

中文

中华中医药学刊

1673-7717

2019

37

5

1110-1114

10.13193/j.issn.1673-7717.2019.05.021

国家自然科学基金青年科学基金项目(81403206) 中国博士后基金面上项目(2014M560646) 湖南省教育厅重点项目(18A209) 湖南省中医药管理局重点项目(201919) 湖南省研究生科研创新项目(CX2017B447).

本校为第一机构

目的:研究熊果酸(UA)对胶原诱导性关节炎(CIA)大鼠外周血淋巴细胞辅助性T细胞17(Th17)/调节性T细胞(Treg)比例失衡的影响.方法:雌性Sprague-Dawley (SD)大鼠,复制CIA模型,造模成功后CIA大鼠随机分为CIA模型组、UA低剂量组、UA高剂量组、甲氨喋呤(MTX)组、UA+ MTX组并予以对应处理.于初次免疫21 d时,收集各组大鼠的外周血淋巴细胞,进行细胞染色,流式细胞术检测外周血Th17/Treg细胞比例,并分离CD;T细胞,RT-PCR法检测RORr、FoxP3、SOCS3mRNA的表达.结果:(1)与空白组相比,模型组CIA大鼠外周血Th17细胞明显增高,与模型组比较,治疗组(UA高剂量组、MTX组、UA+ MTX组)Th17细胞比例明显下调;有显著统计学...

Objective:To investigate the effect of ursolic acid(UA) on the imbalance of peripheral T lymphocyte T helper 17(Th17)/regulatory T cell(Treg) in peripheral blood lymphocytes of rats with collagen-induced arthritis(CIA).Methods: Female Sprague-Dawley(SD) rats were selected and duplicated with CIA model. After successful modeling, CIA rats were randomly divided into CIA model group, UA lowdose group and UA highdose group, Methotrexate(MTX) group, UA+MTX group and corresponding treatment. Om the 21 st day of primary immunization, peripheral blood ...