期刊论文

The Effect of Polygonatum Odoratum Polysaccharide on Cognitive Impairment of D-galactose-induced Aging Mice

中文

中华中医药学刊

1673-7717

2020

38

11

254-258,后插44-后插46

10.13193/j.issn.1673-7717.2020.11.061

湖南省自然科学基金(2019JJ50445) 湖南省教育厅优秀青年项目(16B194) 湖南省研究生创新课题一般项目(CX2018B519)。

本校为第一机构

目的 研究玉竹多糖对D-半乳糖诱导的衰老模型小鼠认知障碍的改善作用.方法 将50只C57小鼠随机分为正常组、衰老模型组、阳性药物组、玉竹多糖低剂量组(1 g/kg)和玉竹多糖高剂量组(2 g/kg),给正常组每天皮下注射生理盐水(10 mL/kg),模型组及药物组小鼠每日皮下注射D-半乳糖(150 mg/kg),同时正常组及模型组每日灌胃同体积生理盐水,药物组小鼠灌胃同体积相应剂量的药物.给药8周后,进行Morris水迷宫测试,随后收集海马组织,测定脑组织中的超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、丙二醛(MDA)及炎症因子IL-1β、IL-6、TNF-α的含量,对脑海马组织进行HE染色、尼氏染色及免疫组化,观察脑组织海马区病...

Objective To study the effect of Polygonatum odoratum polysaccharide on the cognitive impairment of D-galactose-induced aging model mice. Methods Fifty C57 mice were randomly divided into the normal group, aging mice model group, positive medicine group, high(2 g/kg) and low(1 g/kg) dose groups of Polygonatum odoratum polysaccharide. The normal group was subcutaneously injected with normal saline(10 mL/kg) every day, while the mice in the model group and the drug groups were subcutaneously injected with D-galactose(150 mg/kg) every day. Besides...