期刊论文

中文

中华中医药杂志

1673-1727

2009

24

5

650-653

中国博士后科学基金（No.2003033474,No.20060390891）； 湖南省中医药管理局（No.6302-23）；

本校为其他机构

目的:探讨动脉粥样硬化(atheroselerosis,AS)大鼠"痰瘀"病理演变规律和形成机制.方法:复制AS模型大鼠,通过光镜、电镜观察主动脉组织学改变;运用RT-PCR法检测相关基因表达.结果:随着AS的进展,血脂增高、血液流变学异常逐渐加重;在造模第17天脂质代谢明显异常,造模第22天血液流变学显著异常,第32天动脉硬化程度达到2级;基因c-myc、PDGF-A、c-fos、Bcl-2 mRNA表达量依次上调,p53 mRNA表达下调.结论:基因c-myc、PDGF-A、c-fos、Bcl-2mRNA表达上调、p53 mRNA表达下调可能是AS"痰瘀"病理演变的分子基础.

Objective： To explore the molecular mechanisms of atherosclerosis （AS）. Methods： AS rat model was made, arterial morphological histology changes were observed under optic and electronic microscope, and some genes were detected by RT-PCR. Results： There was biochemical change in the blood stasis-phlegm syndrome, which was associated with the alterations of tissue structure. Fat metabolism was obviously abnormal on the 17th day after establishing model in rats.Blood rheology was obviously abnormal on the 22th day.Arteriosclerotic degree reac...