[Yang, Sheng-Hui] Department of Prevention Medicine and Pathogenic Biology, Medical School, Hunan University of Chinese Medicine, Changsha 410208, China;[Lu, Fang-Guo] Department of Prevention Medicine and Pathogenic Biology, Medical School, Hunan University of Chinese Medicine, Changsha 410208, China. firstname.lastname@example.org;[Qin, Lu-Lu] Department of Prevention Medicine and Pathogenic Biology, Medical School, Hunan University of Chinese Medicine, Changsha 410208, China. email@example.com;[Qin, Lu-Lu] Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha 410078, China. firstname.lastname@example.org;[Luo, Bang-An] Department of Mental Health, Brain Hospital of Hunan Province, Changsha 410007, China. email@example.com
vitamin D deficiency;preterm birth;pregnant women;meta
There are disagreements among researchers about the association between vitamin D deficiency during pregnancy and preterm birth (PTB). Therefore, we conducted a meta-analysis of observational studies to evaluate this association. We performed a systematic literature search of PubMed, MEDLINE and the Cochrane Library through August 2015 with the following keywords: "vitamin D" or "cholecalciferol" or "25-hydroxyvitamin D" or "25(OH)D" in combination with "premature birth" or "preterm birth" or "PTB" or "preterm delivery" or "PTD" or "prematurity". Our meta-analysis of 10 studies included 10,098 participants and found that pregnant women with vitamin D deficiency (maternal serum 25 (OH) D levels < 20 ng/mL) experienced a significantly increased risk of PTB (odds ratio (OR) = 1.29, 95% confidence intervals(CI): 1.16, 1.45) with low heterogeneity (I(2) = 25%, p = 0.21). Sensitivity analysis showed that exclusion of any single study did not materially alter the overall combined effect. In the subgroup analyses, we found that heterogeneity was obvious in prospective cohort studies (I(2) = 60%, p = 0.06). In conclusion, pregnant women with vitamin D deficiency during pregnancy have an increasing risk of PTB.
Two new anthraquinones, 1,3-dihydroxy-5-methoxy-6-methoxymethyl-2-methyl-9,10-anthraquinone (1) and 1,3-dihydroxy-5-methoxy-2,6-bismethoxymethyl-9,10-anthraquinone (2), together with ten known anthraquinone derivatives (3-12), three coumarin derivatives (13-15), and 6-gingerol (16) were isolated from the barks of Morinda citrifolia (Noni) collected in the Yongxing island of Xisha. The structures of compounds (1-16) were determined on the basis of extensive spectroscopic analyses, as well as by comparison with literature reports. The new compounds 1 and 2 were tested for their antiviral, cytotoxic, and antibacterial activities. In the primary bioassays, compounds 1 and 2 displayed weak antiH1N1 activity with IC50 values of 66.1 and 10.5 mu M, respectively. In addition, compound 2 showed weak anti-H3N2 activity with IC50 value of 11.5 mu M, and had weak antimicrobial activity against Staphylococcus aureus with MIC value of 24.5 mu M. (C) 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
[Ning, Yi; Zou, Li; Gao, Qiang] Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China;[Hu, Jue] Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China. firstname.lastname@example.org;[Lu, Fangguo] Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China. email@example.com;[Lu, Fangguo] Key Discipline of Pathogenic Biology, The University Innovation Team of Hunan Province, Changsha, Hunan, 410208, People's Republic of China. firstname.lastname@example.org
[Hu, Jue; Lu, Fangguo] Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China.;[Lu, Fangguo] Key Discipline of Pathogenic Biology, The University Innovation Team of Hunan Province, Changsha, Hunan, 410208, People's Republic of China.
Carboxyfluorescein;Drug-resistant bacteria;16S rRNA;Quenching;Enzymatic reaction;Fluorescence resonance energy transfer;Fluorometry;DNA/RNA hybrids;DNA probe
The authors describe a method for the fluorometric determination of methicillin-resistant Staphylococcus aureus (MRSA) by exploiting target-triggered chain reactions and deoxyribonuclease I (DNase I)-aided target recycling. It is making use of a carboxy-fluorescein (FAM)-labeled single-stranded probe containing two sections. One is complementary to the 5' terminus of the target, while the 3' terminus of the other target is adsorbed on the surface of graphene oxide (GO) via pi-stacking interactions without the target (16S rRNA). This adsorption results in quenching of the fluorescence of the label and protects it from being cleaved by DNase I. However, upon addition of the target, DNA/RNA hybrids are repelled by GO. This leads to fluorescence recovery as measured at excitation/emission wavelengths of 480/514 nm due to a chain reaction that is triggered by the target. The signal is strongly amplified by using DNase I-mediated target recycling. The 16S rRNA of MRSA can be detected by this method in the 1 to 30 nM concentration range, and the detection limit is 0.02 nM. The method was applied to analyze bacterial samples, and the detection limit is as low as 30 CFU . mL(-1). The assay is highly sensitive and selective and in our percpetion has a large potential in diagnosis of drug-resistant bacteria. Graphical abstract Schematic of the graphene oxide-based fluorescent bioassay for Methicillin-resistant Staphylococcus aureus detection by using target-triggered chain reaction and deoxyribonuclease I-aided signal amplification.
[Lu, Fangguo] School of Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China;[Zhang, Ran] School of Medicine, Hunan Normal University, Changsha, Hunan, China;[Fang, Fang; Zhang, Fenghua; Peng, Bo; Chang, Haiyan; Chen, Ze] College of Life Science, Hunan Normal University, Changsha, Hunan, China;[Wang, Fuyan] Department of Immunology, College of Basic Medical Sciences, Central South University, Changsha, Hunan, China;[Chen, Ze] Shanghai Institute of Biological Products, Shanghai, China
[Chen, Ze; Fang, Fang] Hunan Normal Univ, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Chen, Ze] Shanghai Inst Biol Prod, Shanghai, Peoples R China.
Maternally-derived antibodies (MDAs) can protect offspring against influenza virus infection but may also inhibit active immune responses. To overcome MDA- mediated inhibition, active immunization of offspring with an inactivated H5N1 whole-virion vaccine under the influence of MDAs was explored in mice. Female mice were vaccinated twice via the intraperitoneal (IP) or intranasal (IN) route with the vaccine prior to mating. One week after birth, the offspring were immunized twice via the IP or IN route with the same vaccine and then challenged with a lethal dose of a highly homologous virus strain. The results showed that, no matter which immunization route (IP or IN) was used for mothers, the presence of MDAs severely interfered with the active immune response of the offspring when the offspring were immunized via the IP route. Only via the IN immunization route did the offspring overcome the MDA interference. These results suggest that intranasal immunization could be a suitable inoculation route for offspring to overcome MDA interference in the defense against highly pathogenic H5N1 virus infection. This study may provide references for human and animal vaccination to overcome MDA-induced inhibition.
Yang, S. H.（杨胜辉）;Brindley, P. J.;Zeng, Q. R.*;Zeng, T. B.;Li, Y. S.;Zhou, J.;Liu, Y.（刘娱）;Liu, B. Y.（刘碧源）;Cai, L. T.;Wu, C. R.（伍参荣）;Lu, F. G.（卢芳国）;Tan, Z. J.（谭周进）;Lan, L. M.;McManus, D. P.
[Yang, S. H.; Wu, C. R.; Tan, Z. J.; Lu, F. G.] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha, Peoples R China.;[Zeng, Q. R.] Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha, Peoples R China.
12th International Congress of Parasitology ICOPA
AUG 15-20, 2010
[Yang, S. H.;Wu, C. R.;Lu, F. G.;Tan, Z. J.] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha, Peoples R China.
Retroviral transduction of cultured schistosomes was explored in our study. The vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse transcriptase gene (hTERT) as a reporter gene under the control of retroviral long terminal repeat (LTR). Pseudotyped virions were employed to transduce Schistosoma japonicum (Sj) to investigate the utility of retrovirus mediated Sj transgenesis and to investigate the activity as human telomerase as a reporter transgene in schistosomes. The presence of transgene hTERT was detected by PCR. Furthermore, analysis of RNAs from transduced parasites, immunohistochemistry of thin sections and immunoblot analysis revealed expression of hTERT transgene in the transduced worms. These findings indicated that Sj could be effectively transduced by VSVG pseudotyped retrovirus carrying the hTERT gene.