期刊论文

Tan, Z.

中文

应用与环境生物学报

1006-687X

2011

17

6

914-917

10.3724/SP.J.1145.2011.00914

科技部支撑计划课题（No.2006BAD08A08）； 长沙市科技局课题（No.K1104070-31）资助~~；

本校为通讯机构

为提高烟草内生放线菌Y12发酵时产生抑菌物质的产量，通过摇瓶正交优化试验，确定了Y12的三角瓶发酵条件. 发酵培养基组成为：黄豆粉1.5%，蛋白胨1.5%，葡萄糖1.5%，可溶性淀粉1.5%，MgSO4·7H2O 0.05%，（NH4）SO4 0.25%，NaCl 0.4%，KH2PO4 0.1%，CaCO3 0.5%；培养基初始pH 7.5，培养温度30 ℃，发酵时间72 h. 经过优化发酵液的抑菌圈直径增加了44.9%. 图2 表2 参17

In order to increase the yield of the antibacterial substance produced by an endophytic actinomycetes, Y 12, from tobacco plant in the process of fermentation, a series of orthogonal experiments on Y 12 were carried out in the shaking flasks to obtain optimal components of the fermentation media as well as the fermentation conditions. It was concluded that the optimal proportion of the fermentation medium was: 1.5% soybean flour, 1.5% peptone, 1.5% glucose, 1.5% soluble starch, 0.05% MgSO 4-7H 2O, 0.25% (NH 4)SO 4, 0.4% NaCl, 0.1% KH 2PO 4 and ...