期刊论文

Effects of astragaloside IV combined with ginsenoside Rg1 on autophagy and PI3K signaling pathways of PC12 cells induced by oxygen glucose deprivation / reoxygenation

中文

中国病理生理杂志

1000-4718

2016

32

11

2003-2009

10.3969/j.issn.1000-4718.2016.11.014

湖南省科技厅一般项目（No.2014SK3001） 中西医结合防治心脑血管疾病的相关基础研究 “中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金

本校为第一机构

目的:探讨黄芪甲苷和人参皂苷Rg1配伍对PC12细胞氧糖剥夺后再复糖复氧(oxygen glucose deprivation /reoxygenation,OGD/R)诱导的细胞自噬的影响及作用机制。方法:以PC12细胞建立OGD/R自噬性损伤模型,观察黄芪甲苷与人参皂苷Rg1配伍对细胞自噬的影响,并通过PI3K Ⅰ/Akt /mTOR和PI3K Ⅲ/beclin-1 / Bcl-2信号通路研究黄芪甲苷与人参皂苷Rg1配伍的作用机制。结果:氧糖剥夺2 h复糖复氧24 h后,PC12细胞内LC3-Ⅱ/LC3-Ⅰ蛋白比值增加。黄芪甲苷、人参皂苷Rg1及黄芪甲苷与人参皂苷Rg1配伍均能下调OGD/R后PC12细胞LC3-Ⅱ/LC3-Ⅰ蛋白比值,且配伍组的效应强于药物单用组。机制研究表明,人参皂苷Rg1单用及黄...

AIM： To probe the effect and the mechanism of astragaloside IV and ginsenoside Rg1 on autophagy of PC12 cells induced by oxygen glucose deprivation / reoxygenation（ OGD / R）. METHODS： The autophagy injury model of PC12 cells induced by OGD / R was established（ PC12 cells were exposed to 2 h of OGD followed by 24 h of reoxygenation）. The effects of astragaloside IV combined with ginsenoside Rg1 on autophagy of PC12 cells were observed,and the mechanism was studied through PI3 K Ⅰ / Akt / m TOR and PI3 K Ⅲ / becline-1 / Bcl-2 signaling pa...