期刊论文

Effects of grub extracts on the expression of retinal ET-1 of rabbits＇ retina vein obstruction model

中文

湖南中医药大学学报

1674-070X

2012

32

9

8-11

10.3969/j.issn.1674-070X.2012.09.002.008.04

湖南省自然科学基金资助项目（06JJ50056）； 湖南省科技厅科研基金资助项目（02ssy3096）； 湖南省教育厅科研基金资助项目（09C729）； 湖南省中医药科研基金资助项目（2010012）； 国家中医药管理局《中医眼科学》重点学科建设项目； 湖南省教育厅《中医五官科学》重点学科建设项目；

本校为第一机构

目的 观察蛴螬提取物对兔视网膜静脉阻塞(retinal vein occlusion,RVO)模型ET-1表达的干预作用.方法 取健康兔36只,每组9只,分为空白组、模型组、血栓通组、蛴螬组4组.采用波长532 nm的Nd:YAG激光光凝法建立RVO模型,连续灌胃给药,实验后7、14、21 d分别处死兔取材,石蜡包埋切片,用免疫组化法检测视网膜组织中ET-1表达活性,计算机图像分析系统并进行统计分析.结果 蛴螬组7、14、21 d与模型组比较,ET-1表达明显下降,差异有统计学意义(P＜0.05)；蛴螬组与血栓通组比较,差异无统计学意义(P＞0.05)；蛴螬组与空白组比较,7、14 d的ET-1表达活性明显高于空白组,差异有统计学意义(P＜0.05),21 d时的ET-1...

Objective To observe the effects of grub extract on the expression of retinal ET-1 of rabbits＇ retina vein obstruction model. Method 36 healthy rabbits were randomly divided into 4 groups as follows： blank group, modeling group, xueshuantong group and grub group, 9 rabbits in each group. RVO models were made with Nd： YAG laser photocoagulation whose wavelength is 532 nm, administered intragastrically continuously. The rabbits were executed respectively in the 7th, 14th and 21st days after the treatment, taking the rabbits retinal tissues and...